FIELD: measurement equipment.
SUBSTANCE: invention relates to a measuring method of variations of fluorescence intensity of voltage-sensitive fluorochrome depending on a potential gradient or ionic strength, which involves addition to the voltage-sensitive fluorochrome of an ionising compound to induce variation of potential or ionic strength, as well as addition of vitamin E and/or cholesterol to increase variation of potential or ionic strength as to voltage-sensitive fluorochrome. Besides, the invention relates to a potential measuring method of actions of cultivated cardiomyocytes.
EFFECT: invention provides measurement of fluorescence intensity of voltage-sensitive fluorochromes or voltage-dependent quantitative variations of their fluorescence intensity without using any such materials (membrane carriers), as cells or two-layer lipidic liposomes.
10 cl, 3 ex, 5 dwg
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Authors
Dates
2014-12-20—Published
2010-10-29—Filed