FIELD: chemistry.
SUBSTANCE: invention relates to engineering enzymology, molecular biology and biotechnology, in particular to increase of efficiency of biocatalytic conversions by means of applying mutant forms of enzymes with improved catalytic properties. In claimed invention increase of efficiency of fermentative synthesis of peptide bond is achieved by application of version of penicillin acylase from E.coli, containing substitution of amino acid residue of enzyme beta-chain in position 484.
EFFECT: application of said penicillin acylase mutant makes it possible to increase output and rate of accumulation of target product in reactions of peptide synthesis, including beta-lactam antibiotics, containing peptide bond in side chain with nucleus.
3 cl, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| E.coli PENICILLIN ACYLASE MUTANT WITH IMPROVED PROPERTIES | 2012 |
|
RU2564578C2 |
| METHOD TO IMPROVE CATALYTIC PROPERTIES OF PENICILLIN ACYLASE | 2009 |
|
RU2575304C2 |
| METHOD OF CATALYTIC PROPERTIES IMPROVEMENT OF PENICILLINACYLAZE FROM ESCHERICHIA COLI AND USE OF MUTANT PENICILLINACYLAZE | 2013 |
|
RU2576002C2 |
| RECOMBINANT STRAIN Escherichia coli-PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS FROM Xanthomonas rubrilineans, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2499830C1 |
| STRAIN Escherichia coli - PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS FROM Xanthomonas rubrilineans, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2502797C1 |
| MODIFIED RAS GENE OF ESCHERICHIA COLI BACTERIUM, CODING PRECURSOR OF ENZYME WITH PENICILLIN G ACYLASE ACTIVITY, RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF ACYLASE PENICILLIN G AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF THIS ENZYME | 2015 |
|
RU2624022C1 |
| METHOD FOR OBTAINING HETEROGENIC BIOCATALYST BASED ON HYDROLASE OF ESTERS OF ALPHA AMINOACIDS, HETEROGENIC BIOCATALYST OBTAINED BY SUCH METHOD, AND SYNTHESIS METHOD OF AMINOBETA-LACTAM ANTIBIOTIC UNDER ACTION OF THIS HETEROGENIC BIOCATALYST | 2013 |
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RU2535893C1 |
| INDUSTRIAL METHOD FOR MICROBIOLOGICAL SYNTHESIS OF PENICILLIN G ACYLASE ESCHERICHIA COLI ENZYME | 2020 |
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RU2729410C1 |
| METHOD FOR PRODUCTION OF HETEROGENEOUS BIOCATALYST, BIOCATALYST BASED ON HYDROLASE OF ALPHA-AMINOACID ETHERS AND METHOD FOR SYNTHESIS OF AMINOBETA-LACTAM ANTIBIOTIC UNDER ACTION OF THIS BIOCATALYST | 2008 |
|
RU2381273C2 |
| METHOD OF SYNTHESIS OF BETA-LACTAM DERIVATIVE | 1991 |
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RU2136759C1 |
