FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology. The invention represents a mutant of penicillin acylase (Penicillin G acylase) from E.coli, containing a substitution of a residue of asparaginic acid of a beta-chain enzyme in position 484 (numeration starts with the first amino acid residue of the beta-chain, containing 557 amino acid residues) with an asparagine residue.
EFFECT: invention makes it possible to obtain penicillin acylase with improved catalytic properties.
2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF SYNTHESISING PEPTIDES, INCLUDING BETA-LACTAM ANTIBIOTICS WITH APPLICATION OF PENICILLIN ACYLASE VERSION | 2012 |
|
RU2537845C2 |
| METHOD TO IMPROVE CATALYTIC PROPERTIES OF PENICILLIN ACYLASE | 2009 |
|
RU2575304C2 |
| METHOD OF CATALYTIC PROPERTIES IMPROVEMENT OF PENICILLINACYLAZE FROM ESCHERICHIA COLI AND USE OF MUTANT PENICILLINACYLAZE | 2013 |
|
RU2576002C2 |
| RECOMBINANT STRAIN Escherichia coli-PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS FROM Xanthomonas rubrilineans, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2499830C1 |
| STRAIN Escherichia coli - PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS FROM Xanthomonas rubrilineans, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2502797C1 |
| MODIFIED RAS GENE OF ESCHERICHIA COLI BACTERIUM, CODING PRECURSOR OF ENZYME WITH PENICILLIN G ACYLASE ACTIVITY, RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF ACYLASE PENICILLIN G AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF THIS ENZYME | 2015 |
|
RU2624022C1 |
| METHOD FOR OBTAINING HETEROGENIC BIOCATALYST BASED ON HYDROLASE OF ESTERS OF ALPHA AMINOACIDS, HETEROGENIC BIOCATALYST OBTAINED BY SUCH METHOD, AND SYNTHESIS METHOD OF AMINOBETA-LACTAM ANTIBIOTIC UNDER ACTION OF THIS HETEROGENIC BIOCATALYST | 2013 |
|
RU2535893C1 |
| RECOMBINANT DNA, WHICH CODES FUNCTIONALLY ACTIVE HYBRID PROTEIN GL7ACA-ACYLASE WITH CHITIN-BINDING DOMAIN (BrdGL7ACA-cbd) AND RECOMBINANT PLASMID pSVH0108, PROVIDING FOR ITS SYNTHESIS IN CELLS Escherichia coli, RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pSVH0108-PRODUCER BrdGL7ACA-cbd | 2006 |
|
RU2388826C2 |
| INDUSTRIAL METHOD FOR MICROBIOLOGICAL SYNTHESIS OF PENICILLIN G ACYLASE ESCHERICHIA COLI ENZYME | 2020 |
|
RU2729410C1 |
| METHOD FOR PRODUCTION OF HETEROGENEOUS BIOCATALYST, BIOCATALYST BASED ON HYDROLASE OF ALPHA-AMINOACID ETHERS AND METHOD FOR SYNTHESIS OF AMINOBETA-LACTAM ANTIBIOTIC UNDER ACTION OF THIS BIOCATALYST | 2008 |
|
RU2381273C2 |
