FIELD: biotechnologies.
SUBSTANCE: carrier is arranged in a column of a test system in the form of a layer of immunoaffinity gel, which is fixed between two porous membranes and provided with grafted - covalently bound - molecules of a toxic agent; the carrier - the gel layer is treated with a blocking solution for closure on the carrier of the rest free places of nonspecific binding; test specimens containing a certain amount of pre-introduced antibodies specific to a toxicant are added; the carrier is treated with a conjugate-containing solution, and namely a conjugate solution of anti-species antibodies chemically bound to luminescent quantum dots or to liposomes containing luminescent quantum dots, and a level of toxicants is determined by illumination of the treated carrier with excitation radiation as to intensity of luminescence excited at quantum dots.
EFFECT: group of inventions allows effective and reliable determination of a level of toxicants in water, food products or physiological liquids.
4 cl, 1 dwg, 3 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR DETERMINING LEVEL OF TOXICANTS IN WATER, FOOD PRODUCTS OR PHYSIOLOGICAL LIQUIDS, AND TEST SYSTEM | 2012 |
|
RU2508553C1 |
METHOD TO DETERMINE LEVEL OF TOXICANTS IN WATER, FOOD OR PHYSIOLOGICAL LIQUIDS AND TEST SYSTEM | 2012 |
|
RU2506586C1 |
METHOD OF DETERMINING LEVEL OF TOXICANTS IN WATER, FOOD PRODUCTS OR BODY FLUIDS AND TEST SYSTEM | 2013 |
|
RU2547577C1 |
NANOCOMPOSITE MATERIAL | 2007 |
|
RU2332352C1 |
METHOD OF NANOCOMPOSITE OPTICAL PROPERTIES CONTROL | 2007 |
|
RU2332697C1 |
HERPES SIMPLEX VIRUS DIAGNOSTICS METHOD | 2018 |
|
RU2702000C2 |
METHOD OF OBTAINING MEDICATION | 2008 |
|
RU2395268C2 |
METHOD FOR MARKING ORGANIC FUELS USING QUANTUM DOTS | 2022 |
|
RU2780550C1 |
METHOD FOR MANUFACTURING LUMINESCENT SENSOR MATERIAL AND LUMINESCENT SENSOR DEVICE FOR THE ANALYSIS OF ACIDIC AND BASIC COMPONENTS IN GAS PHASE | 2017 |
|
RU2758182C2 |
GOLD-COPPER ALKYNYL PHOSPHINE COMPLEXES AS LUMINESCENT LABELS FOR FLUORESCENCE MICROSCOPY | 2013 |
|
RU2541794C1 |
Authors
Dates
2015-01-10—Published
2013-08-21—Filed