METHOD OF DETERMINING LEVEL OF TOXICANTS IN WATER, FOOD PRODUCTS OR BODY FLUIDS AND TEST SYSTEM Russian patent published in 2015 - IPC G01N33/02 G01N33/18 

FIELD: biotechnology.

SUBSTANCE: method of determining the level of toxicants in water, food products or body fluids by the test system of enzyme-linked immunosorbent assay held in the column, consists in that in the column of the test system a carrier is placed, which is used as an activated solid phase of physisorption - activated porous substrate with grafted covalently bonded molecules of toxicant, the carrier is treated with the blocking solution for closing on the carrier of free places of nonspecific binding, the test samples are added, which contain a certain amount of previously added antibodies specific to toxicants, at that the carrier is treated with conjugate-containing solution, which is used as the conjugate solution of antispecies antibodies chemically bound to luminescent quantum dots or with liposomes containing luminescent quantum dots, and the level of the toxicants is determined by lighting of the treated carrier by excitation radiation on the intensity of luminescence excited in the quantum dots. Test system for the method comprises a column in which a carrier in the form of an activated solid phase of physisorption is mounted - activated porous substrate with grafted covalently bound toxicant molecules, at that the column is provided with a device for measuring the level of luminescence, comprising a source of excitation radiation and a photodetector, at that in front of the photodetector a focusing optical system is additionally mounted, and the output of the photodetector is electrically connected through the signal amplifier and the analogue-digital converter to the control unit - a controller, which output is connected to the display unit and through the stabilisation unit to the excitation radiation source. The side walls of the column are made of material transparent for exciting and luminescent radiation.

EFFECT: invention improves the efficiency and accuracy of determining.

4 cl, 3 ex, 1 dwg