METHOD TO DETERMINE LEVEL OF TOXICANTS IN WATER, FOOD OR PHYSIOLOGICAL LIQUIDS AND TEST SYSTEM Russian patent published in 2014 - IPC G01N33/02 G01N33/18 

FIELD: measurement equipment.

SUBSTANCE: invention relates to the field of biotechnology and may be used to determine level of toxicants in water, food or physiological liquids. The method is carried out by performance of enzyme immunoassay in a column of a test system, and this enzyme immunoassay includes placement of a carrier with inoculated anti-species antibodies in the column, treatment of the carrier with a blocking solution, immobilisation of specific antibodies on the carrier, introduction of tested samples, treatment of the carrier with a conjugate-containing solution and analysis of the treated carrier. The carrier is an activated porous substrate with inoculated anti-species antibodies, and the conjugate-containing solution - the solution of conjugate of antigen - toxicant, chemically linked to luminescent quantum points or with liposomes containing luminescent quantum points. The level of toxicants is determined by intensity of luminescence excited in quantum points during illumination of the treated carrier by exciting radiation. The test system for this method includes a column, which is equipped with a device to measure the level of luminescence including a source of exciting radiation and a photodetector. In front of the photodetector there is additionally a focusing optical system, and the output of the photodetector is electrically connected via a signal amplifier and an analog-digital converter to a controller, to the outlet of which an indication unit and a source of exciting radiation are connected.

EFFECT: invention increases efficiency and validity of determination.

4 cl, 3 ex, 1 dwg