PROTEIN EXPRESSION SYSTEMS Russian patent published in 2015 - IPC C12N15/82 A01H5/00 

FIELD: medicine.

SUBSTANCE: invention refers to biochemistry, particularly to a gene expression construct containing: (a) an expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, b) a polylinker for facilitating a gene insertion into a gene expression system. What is disclosed is a gene expression vector containing the above construct and heterologous gene. What is also described is the gene expression vector containing: (a) the expression enhancer sequence produced of RNA-2 genome segment of two-component RNA-virus of the family Comoviridae, (b) the heterologous gene, which codes a protein of interest, as well as a method for expressing the protein of interest with using it. A method for increasing translation enhancement activity of the heterologous protein of interest from the heterologous gene coding the above protein, a method for translation enhancement of the heterologous protein of interest from the heterologous gene or a heterologous open reading frame coding the above protein are disclosed. There are also described a host organism transformed by the above vector, as well as a method for producing the protein of interest, involving collection of the host organism tissue.

EFFECT: invention enables enhancing translation of the heterologous protein of interest from the heterologous gene.

26 cl, 15 dwg, 4 tbl, 6 ex