METHOD FOR PREPARING PERTUSSIS COMPONENT OF COMBINED VACCINES Russian patent published in 2015 - IPC C12N1/20 C12Q1/02 A61K39/10 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology and concerns a method for preparing a pertussis component of complex vaccines. The presented method involves the B. pertussis culture growth on dense Bordet-Gengou and/or CCA nutrient media, the grown colonies selection according to the morphological characteristics, the selected colonies passage, the bacterial mass growth, the culture washout, the extinction reduction of the prepared pertussis suspensions to 10 international optical units, the agglutination reaction to measure agglutinogens 1, 2, 3 and the selection of pertussis suspensions, wherein the agglutinogen content is determined by type-specific serum dilution 1:3200 and more. The detected B. pertussis culture expressing agglutinogens 1, 2, 3 actively after the lyophilisation is used to prepare the pertussis component of complex vaccines.

EFFECT: characterised invention enables preparing the potent pertussis component of complex vaccines.

1 dwg, 5 tbl, 1 ex