FIELD: biotechnologies.
SUBSTANCE: method involves cultivation of pertussis bacteria in liquid growth medium, separation of supernatant from microbial mass by centrifugation, separation of pertussis toxin from it, its detoxification with formalin in presence of saccharose in a thermostat, and sorption of anatoxin on an aluminium hydroxide gel. Strain Bordetella pertussis No. 287 is used as a producer of pertussis toxin. Detoxification of the above toxin Bordetella pertussis is performed during 19-20 days. The obtained anatoxin is combined with anatoxins of vaccine strains No. 203 and No. 305; they are taken in the ratio of 1:0.5:0.5 respectively; a physiological solution with a phosphate buffer is added to the mixture, and the mixture of anatoxins is absorbed on the aluminium hydroxide gel.
EFFECT: invention allows improving activity and reducing toxicity of the obtained cell-free pertussis vaccine.
3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| FRESHLY ISOLATED STRAIN OF BORDETELLA PERTUSSIS BACTERIA - PRODUCER OF A COMPLEX OF PROTECTIVE ANTIGENS FOR PRODUCTION OF CELL-FREE PERTUSSIS VACCINE | 2018 |
|
RU2689903C1 |
| METHOD FOR PRODUCTION OF Bordetella pertussis ANATOXIN | 2005 |
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| METHOD FOR OBTAINING BORDETELLA PERTUSSIS ANATOXIN | 1986 |
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| NEWLY RECOVERED BORDETELLA PERTUSSIS BACTERIA STRAIN - PERTUSSIS TOXIN PRODUCER | 2009 |
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| METHOD FOR SEROLOGICAL ASSESSMENT OF Bordetella pertussis ANATOXIN TOXICITY | 2012 |
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| METHOD FOR PRODUCTION OF ASSOCIATED PERTUSIS, DIPHTHERIA, AND TETANUM VACCINE | 2006 |
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| METHOD FOR OBTAINING DIPHTHERIA, TETANUS TOXOIDS, AND PERTUSSIS VACCINE | 2006 |
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| RECOMBINANT PLASMID pPA-OPRF-ATOX-OPRI DNA, WHICH ENCODES SYNTHESIS OF HYBRID RECOMBINANT PROTEIN, INCLUDING AMINO ACID SEQUENCES OF PROTEIN F AND I OF EXTERNAL MEMBRANE AND ATOXIC VARIANT OF A PSEUDOMONAS AERUGINOSA EXOTOXIN, ESCHERICHIA COLI PA-OPRF-ATOX-OPRI STRAIN - PRODUCER OF HYBRID RECOMBINANT PROTEIN AND METHOD OF OBTAINING SPECIFIED PROTEIN | 2017 |
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