FIELD: medicine.
SUBSTANCE: invention refers to physiology, cryobiology and medicine, namely to human blood nuclear cell preservation technique with the use of inert gas. The method involves cell saturation in Compoplast 300 plasticised container placed in a metal cryobarocontainer with inert gas, xenon at pressure 0.6 atm for 20min in the room temperature environment of 21±2°C; excessive gas is eliminated, and the biological object is removed from the metal container and frozen to -28°C in ethanol, placed into an electric freezer at -80°C. The bioobject is unfrozen in a water bath at 39±1.5°C for 1.5 min.
EFFECT: implementing the invention provides the reliable cryonic preservation of leukocytes in the inert gas medium and the high level of qualitative and morphological safety of leukocyte concentrate of human blood.
1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CRYOPROTECTIVE SOLUTION FOR LEUKOCYTE FREEZING AT LOW TEMPERATURE | 2004 |
|
RU2290808C2 |
| CRYOPROTECTIVE SOLUTION FOR MODERATELY HYPOTHERMIC LEUKOCYTE PRESERVATION (-40°C) | 2009 |
|
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| METHOD FOR HUMAN NUCLEATED BLOOD CELLS CRYOPRESERVATION AT TEMPERATURE OF MINUS 80°C | 2017 |
|
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| METHOD FOR UNSTRUCTURED FREEZING PERIPHERAL BLOOD PRECURSOR CELLS AT MINUS 80 °C WITH A HEMICOLITE SOLUTION | 2019 |
|
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| FROST PROTECTION SOLUTION FOR NUCLEAR BLOOD CELL FREEZING | 2011 |
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| DEVICE FOR FREEZING PRESERVING OF CELL SUSPENSIONS UNDER PRESSURE IN INERT GAS ATMOSPHERE - PORTABLE CRYOBAROCONTAINER | 2012 |
|
RU2506748C1 |
| METHOD FOR UNREGULATED FREEZING OF PERIPHERAL BLOOD PRECURSOR CELLS AT TEMPERATURE OF MINUS 80 °C WITH GEMGEL SOLUTION | 2018 |
|
RU2720771C1 |
| CRYOPRESERVING AGENT FOR BLOOD NUCLEOCYTE FREEZING | 2013 |
|
RU2530149C1 |
| COMBINED CRYOPROTECTIVE SOLUTION FOR THROMBOCYTE FREEZING | 2012 |
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| METHOD FOR CRYOPRESERVATION OF HAEMOPOIETIC STEM CELLS | 2014 |
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RU2569834C1 |
