FIELD: medicine.
SUBSTANCE: cryopreservation of haemopoietic stem cells is conducted by adding a shielding solution of the cryoprotector dimethyl sulphoxide into a suspension of nucleated cells with haemopoietic stem cells at temperature +4.0°C, preparing the produced suspension for freezing by cold adaptation at +4.0°C for 10 minutes. The stem cell suspension is frozen to temperature -196°C and further thawed. A cell suspension coolant represents Lab Armor dry antiseptic thermal beads having a pre-set temperature of +4±1°C.
EFFECT: high cryopreservation process level, sterility of the procedure, as well as high stability of the greater number of nucleated blood cells to a vital stain.
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| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR CRYOPRESERVATION OF HAEMOPOIETIC STEM CELLS | 2016 |
|
RU2624214C1 |
| METHOD FOR UNSTRUCTURED FREEZING PERIPHERAL BLOOD PRECURSOR CELLS AT MINUS 80 °C WITH A HEMICOLITE SOLUTION | 2019 |
|
RU2711152C1 |
| METHOD FOR ADRIFT FREEZING OF PERIPHERAL BLOOD PRECURSOR CELLS AT A TEMPERATURE OF MINUS 80 °C WITH A SOLUTION OF HEMODIOLITE | 2019 |
|
RU2716574C1 |
| METHOD FOR CRYOPRESERVATION OF HAEMOPOIETIC UMBILICAL STEM CELLS | 2013 |
|
RU2547426C1 |
| METHOD OF CRYOPRESERVATION OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD | 2009 |
|
RU2416197C1 |
| METHOD FOR REDUCING TOXICITY OF CRYOPRESERVATION SOLUTION BASED ON DIMETHYL SULFOXIDE AFTER THAWING OF HEMATOPOIETIC STEM CELLS | 2020 |
|
RU2744614C1 |
| METHOD FOR HUMAN NUCLEATED BLOOD CELLS CRYOPRESERVATION AT TEMPERATURE OF MINUS 80°C | 2017 |
|
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| COMBINATION CRYOPROTECTOR "DIMETHYL SULPHOXIDE/RHEOPOLYGLUCIN" FOR STEM CELL CRYOPRESERVATION AND METHOD FOR CRYOPRESERVATION THEREOF FOR CLINICAL APPLICATIONS | 2014 |
|
RU2563117C1 |
| METHOD FOR UNRESTRICTED FREEZING PERIPHERAL BLOOD PRECURSOR CELLS AT MINUS 80 °C UNDER PROTECTION OF DIMETHYLSULPHOXID | 2018 |
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RU2707921C1 |
| METHOD FOR CRYOGENIC PRESERVATION OF HAEMATOPOIETIC UMBILICAL CORD STEM CELLS | 2012 |
|
RU2554405C2 |
