METHOD FOR DESIGNING BIOENGINEERED RAT'S LUNG FRAME Russian patent published in 2015 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: method for designing a bioengineered rat's lung frame involves lung perfusion by detergent-enzymatic method with controlling the frame quality by histological method. The lung perfusion is accompanied by continuous atmospheric air pulmonary ventilation according to physiological parameters, through a trachea for 24 hours by means of successive exposure to decellularising solutions. That is ensured by using phosphate buffer, 1% aqueous sodium deoxycholate, swine pancreatic DNAase I, purified water at an equal exposure length. To ensure the quality of the following re-cellularisation, a colorimetric method is used to prove the designed lung frame biocompatible, viable and having its architechtonics unchanged by determining its biomechanical tensile and compression strength, by fixing pulmonary compliance.

EFFECT: maintaining the lung matrix structure and its quality, eliminating the risk of contamination.

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