METHOD FOR MICROSCOPIC EXAMINATION OF NATIVE SMEAR FROM TONGUE ROOT Russian patent published in 2015 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: invention refers to microbiology, namely to a method for the microbiological examination of a native smear from a tongue root mucosa. The substance of the method consists in the fact that a wooden spatula is used to sample biomaterial 0.5 ml by deep scraping from the mucous membrane at the boundary of the posterior one-third of the tongue dorsum and root; two smears are prepared on two slides, 1.5-2.0 cm² in area; the smears are dried out for 20 minutes and fixed by flame heat of an alcohol lamp continuously for 5-6 seconds, Gram-stained by a complicated differential method, microscoped with immersion with the use of a ×100 objective lens; at least 10 visual fields are inspected on each slide taking into account quantitative and qualitative characteristics of microorganisms, cells and nuclei of the epithelium.

EFFECT: invention enables the more precise examination of the native smear of the tongue.

4 dwg, 1 tbl, 2 ex