FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the pharmaceutical industry, namely to a method of obtaining pectins from a biomass of cultured tissues of plants Silene vulgaris (M.) G. The method of obtaining pectins with an increased content of galactose residues in side carbon chains from Calusa cultures includes raw material destruction, extraction with water, processing of the biomass with hydrochloric acid, water washing, extraction with ammonium oxalate solution, sedimentation of polysaccharide with ethanol, dialysis and lyophilising, with Caluse cultures being preliminarily grown for 21 hours on an agarised nutrient medium, which contains the ferment 1,4-β-D-galactozyltranspherase in a specified concentration, and as the raw material the biomass of cultured tissues of plants Silene vulgaris is applied.
EFFECT: method makes it possible to obtain physiologically active pectins, which have a specified structure and stable chemical composition.
1 tbl, 4 ex
Title | Year | Author | Number |
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NUTRIENT MEDIUM FOR CULTIVATION OF CALLUS CULTURE OF SPOTTED HEMLOCK (Conium maculatum L) | 2015 |
|
RU2596402C1 |
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RU2393217C1 |
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RU2448119C2 |
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RU2149642C1 |
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RU2458121C1 |
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RU2296132C2 |
Authors
Dates
2015-05-20—Published
2013-10-22—Filed