FIELD: biotechnology.
SUBSTANCE: method comprises performing PCR with electrophoretic detection of results, the transfer of amplification product on the gel, and assessment of the reaction. At that the study of each sample is carried out in two reactions using primers. In the first reaction the bacterium Pasteurella multocida is detected and the serogroups A and D are genotyped, in the second reaction the serogroups B, E and F are genotyped. The method enables to identify and genotype the strains and isolates of bacterium Pasteurella multocida of five serogroups - A, B, D, E, and F in pure or mixed cultures, as well as directly in samples of biological material from animals. The method may be used in veterinary microbiology for diagnostics of pasteurellosis in farm animals.
EFFECT: increase in the accuracy of diagnostics.
4 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR IDENTIFICATION AND SUBTYPING OF BACTERIAL DNA OF Pasteurella multocida OF SEROTYPES A, B, D, E, F BY PCR METHOD IN REAL TIME | 2014 |
|
RU2551958C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND USING THEM IN METHOD FOR IDENTIFYING SEROGROUP D Pasteurella multocida BACTERIAL STRAINS AND ISOLATES IN CATTLE BY POLYMERASE CHAIN REACTION | 2014 |
|
RU2562167C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF STRAINS AND ISOLATES OF BACTERIA Pasteurella multocida OF SEROGROUP A IN CATTLE AND METHOD OF THEIR APPLICATION | 2013 |
|
RU2527153C1 |
| METHOD TO DETECT PATHOGENIC STRAINS AND ISOLATES OF BACTERIA PASTEURELLA MULTOCIDA | 2012 |
|
RU2477321C1 |
| KIT OF REAGENTS FOR GENETIC TYPING STRAINS OF ANTHRAX AGENT BY FRAGMENT ANALYSIS "OM-SIBERIAN PLAGUE-GENOTYPE" | 2019 |
|
RU2730868C1 |
| METHOD FOR IDENTIFICATION OF VIBRIO-CHLERAE O1 STRAINS, DETERMINATION OF THEIR TOXIGENICITY AND BIOVAR WITH DIFFERENTIATION OF EL TOR BIOVAR ON TYPICAL AND GENETICALLY CHANGED VERSIONS BY MULTIPLEX POLYMERASE CHAIN REACTION AND TEST SYSTEM FOR IMPLEMENTATION THEREOF TAKING INTO ACCOUNT RESULTS IN REAL TIME MODE | 2019 |
|
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| METHOD FOR IDENTIFYING YERSINIA PESTIS AND YERSINIA PSEUDOTUBERCULOSIS AND SIMULTANEOUS DIFFERENTIATION OF YERSINIA PESTIS OF MAIN AND CENTRAL ASIAN SUBSPECIES BY MULTIPLEX PCR | 2020 |
|
RU2737775C1 |
| SET FOR DETECTING LEGIONELLOSIS AGENT IN BIOLOGICAL MATERIAL AND ENVIRONMENTAL MEDIUM | 2009 |
|
RU2397251C1 |
| METHOD FOR IDENTIFICATION OF TOXIGENIC GENETIC VARIANTS OF CHOLERA CAUSATIVE AGENT EL TOR WITH SET OF MUTATIONS IN GENES OF VIRULENCE AND PANDEMIC ISLAND GENES BY MULTIPLEX POLYMERASE CHAIN REACTION | 2022 |
|
RU2815711C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS FT 182 AND A METHOD OF DETERMINING BACTERIA FRANCISELLA TULARENSIS (VERSIONS) | 2019 |
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RU2706564C1 |
