FIELD: biotechnologies.
SUBSTANCE: method is proposed to detect pathogenic strains and isolates of bacteria Pasteurella multocida with the help of PCR. The method may be used in veterinary microbiology for diagnostics of pasteurellosis of farm animals. The method includes isolation of cultures of microorganisms from a pathological material on artificial nutrient media, completion of PCR with synthetic oligonucleotide primers SEQ ID NO:1 - 5' atgatgtcggcatgaatttctcagc 3' and SEQ ID NO:2 - 5' aacatagccagcgccagcaatgt 3'. The product of amplification is transferred to gel, and the completed reaction is assessed. To set PCR, a suspension of microorganisms is used on sterile distilled water without isolation of DNA. PCR is performed in 1 round. In case of positive reaction, a fragment is synthesised, corresponding to the size of 534 bps.
EFFECT: invention makes it possible to efficiently detect pathogenic strains and isolates of a bacteria Pasteurella multocida.
3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF STRAINS AND ISOLATES OF BACTERIA Pasteurella multocida OF SEROGROUP A IN CATTLE AND METHOD OF THEIR APPLICATION | 2013 |
|
RU2527153C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND USING THEM IN METHOD FOR IDENTIFYING SEROGROUP D Pasteurella multocida BACTERIAL STRAINS AND ISOLATES IN CATTLE BY POLYMERASE CHAIN REACTION | 2014 |
|
RU2562167C1 |
| METHOD OF DETECTING AND GENOTYPING BACTERIA Pasteurella multocida OF CATTLE OF SEROGROUPS A, B, D, E, F IN MULTIPLEX POLYMERASE CHAIN REACTION | 2014 |
|
RU2551962C1 |
| METHOD FOR REAL-TIME PCR DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA | 2022 |
|
RU2785435C1 |
| TEST SYSTEM FOR DETECTING DNA OF CAUSATIVE AGENT OF MORAXELLOSIS KPC (MORAXELLA BOVIS) IN BIOLOGICAL MATERIAL OF ANIMALS AND FEED USING POLYMERASE CHAIN REACTION IN REAL TIME | 2023 |
|
RU2814556C1 |
| PASTEURELLA MULTOCIDA "PM - B" BACTERIUM STRAIN FOR MANUFACTURE OF BIOPREPARATIONS FOR SPECIFIC PREVENTION OF PASTEURELLOSIS (HEMORRHAGIC SEPTICEMIA) IN CATTLE CAUSED BY SEROGROUP B PASTEURELLA | 2023 |
|
RU2818959C1 |
| METHOD OF DECTING PASTEURELLA AND/OR Pseudomonas Aeruginosa bacteria | 2008 |
|
RU2391409C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR IDENTIFICATION AND SUBTYPING OF BACTERIAL DNA OF Pasteurella multocida OF SEROTYPES A, B, D, E, F BY PCR METHOD IN REAL TIME | 2014 |
|
RU2551958C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR DETECTING 1A AND 1B VIRUS SUBGENOTYPES OF VIRAL DIARRHOEA - BOVINE MUCOSAL DISEASE AND METHOD OF APPLYING THEREOF | 2009 |
|
RU2409673C1 |
| KIT FOR DIFFERENTIATING CATTLE PESTIVIRUSES AND METHOD FOR DIFFERENTIATING CATTLE PESTIVIRUSES | 2019 |
|
RU2731716C1 |
