FIELD: veterinary medicine.
SUBSTANCE: synthetic oligonucleotide primers are proposed for identification of strains and isolates of the bacterium Pasteurella multocida of the serogroup A in cattle and method of their application. The proposed method comprises carrying out PCR with synthetic oligonucleotide primers for gene region hyaD Pasteurella multocida, transfer of the amplified product on the gel and assessing the reaction. For the PCR setup the DNA is used isolated from pathological material and bacterial cultures. PCR is carried out as 1 round. In case of the positive reaction the fragment is synthesised, corresponding to the size of 218 bps.
EFFECT: method can be used in veterinary microbiology for diagnosing pasteurellosis of farm animals.
2 cl, 5 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND USING THEM IN METHOD FOR IDENTIFYING SEROGROUP D Pasteurella multocida BACTERIAL STRAINS AND ISOLATES IN CATTLE BY POLYMERASE CHAIN REACTION | 2014 |
|
RU2562167C1 |
| METHOD OF DETECTING AND GENOTYPING BACTERIA Pasteurella multocida OF CATTLE OF SEROGROUPS A, B, D, E, F IN MULTIPLEX POLYMERASE CHAIN REACTION | 2014 |
|
RU2551962C1 |
| METHOD TO DETECT PATHOGENIC STRAINS AND ISOLATES OF BACTERIA PASTEURELLA MULTOCIDA | 2012 |
|
RU2477321C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR IDENTIFICATION AND SUBTYPING OF BACTERIAL DNA OF Pasteurella multocida OF SEROTYPES A, B, D, E, F BY PCR METHOD IN REAL TIME | 2014 |
|
RU2551958C1 |
| TEST SYSTEM FOR DETECTING DNA OF CAUSATIVE AGENT OF MORAXELLOSIS KPC (MORAXELLA BOVIS) IN BIOLOGICAL MATERIAL OF ANIMALS AND FEED USING POLYMERASE CHAIN REACTION IN REAL TIME | 2023 |
|
RU2814556C1 |
| METHOD FOR REAL-TIME PCR DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA | 2022 |
|
RU2785435C1 |
| PASTEURELLA MULTOCIDA "PM - B" BACTERIUM STRAIN FOR MANUFACTURE OF BIOPREPARATIONS FOR SPECIFIC PREVENTION OF PASTEURELLOSIS (HEMORRHAGIC SEPTICEMIA) IN CATTLE CAUSED BY SEROGROUP B PASTEURELLA | 2023 |
|
RU2818959C1 |
| METHOD FOR DETECTION AND IDENTIFICATION OF CORONAVIRUSES IN CATTLE | 2021 |
|
RU2766344C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR DETECTING 1A AND 1B VIRUS SUBGENOTYPES OF VIRAL DIARRHOEA - BOVINE MUCOSAL DISEASE AND METHOD OF APPLYING THEREOF | 2009 |
|
RU2409673C1 |
| KIT FOR DIFFERENTIATING CATTLE PESTIVIRUSES AND METHOD FOR DIFFERENTIATING CATTLE PESTIVIRUSES | 2019 |
|
RU2731716C1 |
