FIELD: medicine.
SUBSTANCE: inventions refers to veterinary microbiology and concern oligonucleotide primers for identifying serogroup D Pasteurella multocida bacterial strains and isolates, as well as a method using them. The presented method involves recovering microbial culture from a pathologic material on artificial nutrient media. That is followed by carrying out a polymerase chain reaction with synthetic oligonucleotide primers having the sequences SEQ ID NO:1 - 5' catcgcatccagaatagcaaact 3', SEQ ID NO:2 - 5' ctccgatgctttggttgtg 3' per dcbF gene region responsible for heparosan synthetase synthesis. The amplification product is gel transferred, and the reaction process is assessed. If the reaction appears to be positive, a fragment having a size of 355 base pairs is synthesised.
EFFECT: presented inventions can be used in veterinary microbiology for the purpose of diagnosing pasteurellosis in farm animals.
2 cl, 5 tbl, 1 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF STRAINS AND ISOLATES OF BACTERIA Pasteurella multocida OF SEROGROUP A IN CATTLE AND METHOD OF THEIR APPLICATION | 2013 |
|
RU2527153C1 |
| METHOD OF DETECTING AND GENOTYPING BACTERIA Pasteurella multocida OF CATTLE OF SEROGROUPS A, B, D, E, F IN MULTIPLEX POLYMERASE CHAIN REACTION | 2014 |
|
RU2551962C1 |
| METHOD TO DETECT PATHOGENIC STRAINS AND ISOLATES OF BACTERIA PASTEURELLA MULTOCIDA | 2012 |
|
RU2477321C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR IDENTIFICATION AND SUBTYPING OF BACTERIAL DNA OF Pasteurella multocida OF SEROTYPES A, B, D, E, F BY PCR METHOD IN REAL TIME | 2014 |
|
RU2551958C1 |
| METHOD FOR DETECTING CLOSTRIDIUM SPECIES CLOSTRIDIUM SPOROGENES, CLOSTRIDIUM PERFRINGENS AND CLOSTRIDIUM SORDELLII | 2023 |
|
RU2826158C1 |
| KIT FOR DIFFERENTIATING CATTLE PESTIVIRUSES AND METHOD FOR DIFFERENTIATING CATTLE PESTIVIRUSES | 2019 |
|
RU2731716C1 |
| METHOD FOR DETECTION AND IDENTIFICATION OF CORONAVIRUSES IN CATTLE | 2021 |
|
RU2766344C1 |
| TEST SYSTEM FOR DETECTING DNA OF CAUSATIVE AGENT OF MORAXELLOSIS KPC (MORAXELLA BOVIS) IN BIOLOGICAL MATERIAL OF ANIMALS AND FEED USING POLYMERASE CHAIN REACTION IN REAL TIME | 2023 |
|
RU2814556C1 |
| OLIGONUCLEOTIDE PRIMERS AND METHOD OF DETECTING DNA OF Mycobacterium paratuberculosis THAT IS PARATUBERCULOSIS AGENT BY METHOD OF POLYMERASE CHAIN REACTION (PCR) | 2010 |
|
RU2435852C1 |
| METHOD FOR REAL-TIME PCR DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA | 2022 |
|
RU2785435C1 |
