METHOD OF OBTAINING POPULATION OF CELLS, EXPRESSING PURIPOTENCY MARKERS AND MARKERS, CHARACTERISTIC OF FORMED ENDODERM LINE Russian patent published in 2015 - IPC C12N5/735 C12N5/74 

FIELD: medicine.

SUBSTANCE: claimed invention deals with method of obtaining population of cells, expressing pluripotency markers and markers, characteristic of formed endoderm line. Claimed method includes the following stages (a) obtaining population of cells, expressing markers HNF-3β, GATA-4, Mixl1, CXCR4, and SOX-17, characteristic of formed endoderm line, and (b) cultivation of population of cells under conditions of hypoxia on tissue culture substrate, which is not subjected to preliminary treatment with protein or extracellular matrix, in medium with addition of activin A and ligand Wnt and IGF-1 or insulin, transferrin and selenium, where cultivated cells express markers of formed endoderm line and pluripotency markers.

EFFECT: characterised invention makes it possible to obtain cells, capable of cultivation under conditions of hypoxia, which do not require lines of feeding cells, coatings of complex matrix proteins and preserve differentiation potential.

16 cl, 39 dwg, 9 tbl, 32 ex