SEQUENCE 3'-UTR FOR RNA STABILIZATION Russian patent published in 2020 - IPC C12N15/67 

FIELD: biotechnology.

SUBSTANCE: described is a nucleic acid molecule comprising, in 5' → 3' transcription direction: (a) a promoter, (b) a transcribed nucleic acid sequence or a nucleic acid sequence for introducing a transcribed nucleic acid sequence and (c) a nucleic acid sequence which, when transcribed under the control of promoter (a), encodes 3'-untranslated region of a transcript which is not naturally associated with nucleic acid (b), wherein said 3'-untranslated region comprises a nucleic acid sequence which is selected from a group consisting of: (c-1) a nucleic acid sequence of 3'-untranslated FCGRT region, a fragment thereof or a variant of said nucleic acid or fragment sequence, (c-2) a nucleic acid sequence of 3'-untranslated region of LSP1, a fragment thereof or a variant of said nucleic acid or fragment sequence, (c-3) a nucleic acid sequence of 3'-untranslated region of CCL22, a fragment thereof or a variant of said nucleic acid or fragment sequence, (c-4) a nucleic acid sequence of 3'-untranslated region of AES, a fragment thereof or a variant of said nucleic acid or fragment sequence, (c-5) a nucleic acid sequence of 3'-untranslated region of PLD3, a fragment thereof or a variant of said nucleic acid or fragment sequence, (c-6) a nucleic acid sequence of a non-coding RNA of MTRNR1, a fragment thereof or a variant of said nucleic acid or fragment sequence, (c-7) a nucleic acid sequence of 3'-untranslated region of HLA-DRB4, a fragment thereof or a variant of said nucleic acid sequence or fragment and (c-8) any combination of two or more nucleic acid sequences, fragments and/or variants designated by numbers (c-1), (c-2), (c-3), (c-4), (c-5), (c-6) and (c-7), where the nucleic acid sequences (b) and (c) under the control of the promoter (a) can be transcribed to form a common transcript, in which a nucleic acid sequence transcribed from a nucleic acid sequence (c) is active with respect to increasing transmission efficiency and/or stability of the nucleic acid sequence, transcribed from the transcribed nucleic acid sequence (b).

EFFECT: invention provides high stability of the transcript and/or efficiency of translation.

53 cl, 14 dwg, 18 tbl, 12 ex