METHOD FOR PRODUCING LACTOFERRIN, LACTOFERRIN-CONTAINING FRACTION AND USING IT Russian patent published in 2016 - IPC C07K1/18 C07K14/47 C07K14/79 A61K38/40 A61K35/20 A61P31/04 A61P35/00 A61P17/02 A61P11/00 

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to biotechnology. A raw material is presented by milk, which has not been processed at a temperature of more than 55°C. The above raw material is extracted on cation-exchange resin with using a concentrated solution of sodium chloride. A solution of primary milk protein containing lactoferrin, lactoperoxidase and other foreign matters is produced. The solution of primary milk protein is purified on cation exchange resin balanced with acetate buffer in the concentration of 50 mM at pH from 4 to 9. An elution procedure is ensured with using acetate buffer solutions in the concentration of 50 mM with the different concentrations of the sodium chloride solution - from 0.02 to 1.5 M. A lactoferrin-containing fraction is collected. The fraction contains more than 95% pure lactoferrin and is substantially free from lipopolysaccharides, endotoxins and angiogenin and has an iron saturation level from 9% to 20%. The produced fraction is applicable to accelerate maturation of a newborn's gastrointestinal tract or to recover a post-gastroenteritis intestinal mucosa, to increase monocyte activity and to improve the cytotoxic function of killer cells, as an anti-inflammatory agent, to reduce expression of anti-inflammatory cytokines, to inhibit or eliminate bacteria, to treat the diseases associated with bacterial films, to prepare solutions or ointments for wound cleaning or eye care, and to treat infectious respiratory diseases.

EFFECT: method for producing lactoferrin, the lactoferrin-containing fraction and using it are presented.

15 cl, 4 tbl, 11 dwg