METHOD OF ESTIMATING LOCAL COLD INJURY IN EARLY REACTIVE PERIOD Russian patent published in 2015 - IPC G01N33/48 

Abstract RU 2554821 C1

FIELD: medicine.

SUBSTANCE: invention represents method of estimating degree of local cold injury in early reactive period, including separation of peripheral blood lymphocytes, estimation of expression of injury of plasma membrane of lymphocytes in state of blebbing by means of phase-contrast microscopy counted per 100 cells, calculation of index of lymphocyte blebbing ILB by formula: I L B = terminal blebbing × 100 initial + terminal blebbing ( % ) , obtained ILB index is multiplied by level of aspartate aminotransferase - AST, measured in mmol//h*l, with obtaining frostbite degree coefficient FDC, if FDC values are from 3.96 to 7.7 conv. units, II degree of local cold injury is diagnosed from 7.7 to 17.4 conv. units - III degree, higher than 17.4 conv. units - IV degree.

EFFECT: simplification and increase of objectivity of determining degree of local cold injury in early reactive period.

1 dwg, 1 tbl, 3 ex

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RU 2 554 821 C1

Authors

Jur'Eva Margarita Jur'Evna

Vinnik Jurij Semenovich

Salmina Alla Borisovna

Malinovskaja Natalija Aleksandrovna

Tepljakova Ol'Ga Valerievna

Tret'Jakova Nina Gennad'Evna

Dates

2015-06-27Published

2014-02-18Filed