METHOD OF ESTIMATING LOCAL COLD INJURY IN EARLY REACTIVE PERIOD Russian patent published in 2015 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention represents method of estimating degree of local cold injury in early reactive period, including separation of peripheral blood lymphocytes, estimation of expression of injury of plasma membrane of lymphocytes in state of blebbing by means of phase-contrast microscopy counted per 100 cells, calculation of index of lymphocyte blebbing ILB by formula: $$ILB=\frac{\text{terminal blebbing}\times 100}{\text{initial}+\text{terminal blebbing}}(\%),$$ obtained ILB index is multiplied by level of aspartate aminotransferase - AST, measured in mmol//h*l, with obtaining frostbite degree coefficient FDC, if FDC values are from 3.96 to 7.7 conv. units, II degree of local cold injury is diagnosed from 7.7 to 17.4 conv. units - III degree, higher than 17.4 conv. units - IV degree.

EFFECT: simplification and increase of objectivity of determining degree of local cold injury in early reactive period.

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