CULTURE OF PLURIPOTENT STEM CELLS ON MICROCARRIERS Russian patent published in 2015 - IPC C12N5/735

Abstract RU 2555538 C2

FIELD: medicine.

SUBSTANCE: invention relates to biotechnology. Method includes adhesion of cell population to microcarrier in medium, containing Pho-kinase inhibitor. After that, cultivation of cells, separation of cells from microcarrier and adhesion of obtained cells to second microcarrier in medium containing inhibitor of Rho-kinase. Invention can be used in medicine.

EFFECT: method of growing human embryonic stem cells is disclosed.

7 cl, 48 dwg, 3 tbl, 11 ex

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RU 2 555 538 C2

Authors

Nel'Son, Shelli

Dates

2015-07-10—Published

2009-11-19—Filed