FIELD: pharmacy.
SUBSTANCE: invention relates to a method (versions) of formation and differentiation of cells population in which more than 80% of the cells population expresses markers which are characteristic for the line of formed endoderm. The method comprises the differentiation of population of the human embryonic stem cells in medium DMEM or MCDB-131, containing activin A and ligand Wnt, or GDF-8 and 14-prop-2-en-1-yl-3,5,7,14,17, 23.27-geptaazatetratcyclo[19.3.1.1~2,6~.1~8,12~]geptakoza-1 (25), 2 (27), 3,5,8 (26), 9,11,21,23-nonaene-16-on, where the glucose concentration is not more than 10.5 mmol/l, where embryonic human stem cells are stable cell lines of human embryonic stem cells, stable cell lines of human embryonic germ cells, such as H1 cells, H7 cells H9 cells or BG01V cells.
EFFECT: ability to use the invention in the laboratory to create an in vitro functional insulin-expressing cell, which would be closer to β-cell.
10 cl, 9 dwg, 2 tbl, 4 ex
