FIELD: embryology.
SUBSTANCE: present invention relates to embryology. Method for producing a population of pancreatic endocrine precursor cells from of pluripotent stem cells which are human embryonic stem cells of line H9, H1, H7 and human embryonic stem cells of line SA002 consists of the following stages: the culturing of a population of the said pluripotent stem cells; the differentiation of the population of the said pluripotent stem cells into a population of marker-positive cells, specific for a formed endoderm line; the differentiation of the population of marker-positive cells, specific for a formed endoderm line, into a population of gut cells; the differentiation of the population of gut cells into a population of the cells of the rear segment of the foregut and the differentiation of the population of cells of the rear segment of the foregut into a population of pancreatic endocrine precursor cells by culturing the population of the cells of the rear segment of the foregut in a medium with the addition of CYP26A inhibitor and without adding retinoic acid.
EFFECT: this method increases the efficiency of differentiation.
18 cl, 3 ex, 5 dwg
