FIELD: chemistry.
SUBSTANCE: invention relates to a method of producing immunoglobulins of subclass IG1 and IG4 from cultivated cell supernatants. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution, host cell nucleic acids and proteins can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.
EFFECT: obtaining immunoglobulins of subclass IG1 and IG4.
13 cl, 12 dwg, 13 tbl, 2 ex
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