ANTIBODIES WITHOUT Fc-FRAGMENT INCLUDING TWO FAB-FRAGMENT AND METHODS OF APPLICATION Russian patent published in 2017 - IPC C07K16/46 C12P21/08 A61K39/395 

FIELD: medicine, pharmacy.

SUBSTANCE: group of inventions refers to biotechnology, namely to bispecific antibodies, and application thereof. The bispecific antibodies comprise two Fab fragments, the first Fab fragment comprises at least one antigen-binding site specific for the first antigen and the second Fab fragment comprises at least one antigen-binding site specific for the second antigen, where variable regions or constant regions between heavy and light chains of the second Fab fragment are exchanged, at that, the bispecific antibody lacks the Fc domain. A method and a host cell for their preparation as well as a pharmaceutical composition and an immunoconjugate for cancer treatment containing such bispecific antibodies are also proposed. The advantage of the claimed bispecific antibodies is the reduced fragments aggregation, improved stability, and improved efficiency of binding to antigen.

EFFECT: invention allows effective cancer therapy.

13 cl, 23 dwg, 2 tbl, 9 ex