SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES AND METHOD FOR DETECTING BOVINE PESTIVIRUS H Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention discloses synthetic oligonucleotide primers and probes for detecting RNA of bovine pestivirus H and mRNA of the bovine GAPDH gene as a control of the reaction efficiency and a method for use thereof. Proposed method involves isolation of RNA from samples of embryonal serums and biomaterial, reverse transcription and multiplex polymerase chain reaction with synthetic oligonucleotide primers and a probe and evaluation of reaction on 2 channels. Recorded fluorescence on the FAM channel indicates the detection of pseudocolitis H in the sample, and the glow on the HEX channel - on detecting mRNA of the bovine GAPDH, wherein detecting mRNA of bovine GAPDH is used as a control of reaction efficiency.

EFFECT: method can be used in veterinary science to detect possible contamination of embryonal serums used for culturing cell cultures and producing biopreparations, bovine pestivirus H, as well as for diagnosing infectious diseases of farm animals, particularly an infection caused by bovine pestivirus H.

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