FIELD: medicine.
SUBSTANCE: invention relates to medicine and deals with method of creating nanosized diagnostic label based on conjugates of nanoparticles and single domain antibodies, and can be applied for production of new generations of diagnosticums. Method includes preparing surface of nanoparticles by means of polyethyleneglycol derivatives, hexahistidine sequence of amino acids with terminal cysteine residue is introduced into C-terminal part of single domain antibody, nanoparticles conjugate with single domain antibodies with recognising centres in such a way that recognising centres of single domain antibodies are always oriented towards location of analysed molecules.
EFFECT: invention provides highly sensitive detection of analysed molecules of biological disease markers in biological liquids and tissues.
20 cl, 3 dwg
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