FIELD: medicine.
SUBSTANCE: technique involves sampling a biopsy material from a skin growth of interest, carrying out real-time PCR of the sampled material to measure a relative microRNA expression level as shown by snRNA-U6 normalising microRNA with hsa-miR-205 gene taken as a differential diagnostic marker. If the relative expression level falls within the range of 0.29926 to 13.202, the analysing skin growth is considered to be melanoma; the relative expression level from 25.70993 to 1583.114 shows a nevus; an intermediate range of values falling within 13.202 to 25.70993 requires performing an immunohistochemical study of the biopsy material with using primary antibodies against the specific markers: S 100, HMB 45, MART 1, MELAN A, CD-63, MITF.
EFFECT: using the invention enables optimising and accelerating the differential diagnosis of melanocytic skin growths.
1 tbl, 3 ex
Title | Year | Author | Number |
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METHOD OF SIMULTANEOUS VERIFICATION AND PREDICTION OF COURSE OF UVEAL MELANOMA | 2006 |
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METHOD OF SIMULTANEOUS VERIFICATION OF UVEAL MELANOMA COURSE FORECAST | 2006 |
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RU2327420C1 |
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DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR JUVENILE NEVUS | 2008 |
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METHOD FOR DIFFERENTIAL DIAGNOSIS OF GLIOMAS OF HUMAN BRAIN | 2015 |
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RU2583871C1 |
METHOD FOR DIFFERENTIAL DIAGNOSIS OF GLIOMAS BASED ON ANALYSIS OF GENE EXPRESSION AND MICRO-RNA | 2018 |
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METHOD FOR DIFFERENTIAL DIAGNOSTICS OF MELANOCYTIC SKIN GROWTHS | 2016 |
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RU2635772C1 |
METHOD FOR DIAGNOSING GLIAL BRAIN TUMORS OF HIGH DEGREE OF MALIGNANCY | 2020 |
|
RU2742413C1 |
METHOD FOR NON-INVASIVE DIAGNOSTICS OF SKIN PIGMENT FORMATIONS | 2021 |
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RU2773093C1 |
HUMAN SKIN MELANOMA CELL LINE mel-Alx-LP | 2022 |
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RU2796357C1 |
Authors
Dates
2015-08-20—Published
2014-06-09—Filed