DIAGNOSTIC TECHNIQUE FOR SECONDARY IMMUNODEFICIENT DISEASES RELATED TO CHEMICAL CONTAMINANT Russian patent published in 2012 - IPC G01N33/53 C12Q1/68 

FIELD: medicine.

SUBSTANCE: oropharyngeal or nasal biological material is sampled to recover DNA; it is followed by the PCR with using three primers simultaneously: Epstein-Barr gene (EBV) LMP, Cytomegalovirus gene (CMV) MIE, type 6 human herpes virus gene pol (HHV-6). A human 3-globin gene is used as an internal reference sample (IRS). Data derived from the PCR amplification are used to calculate the quantitative content of specific viral DNA in the analysed sample by formula. An index is determined for each viral DNA, while the blood sample is analysed for the quantitative content of a chemical contaminant technogenically significant in the territory; it is followed by stating the presence or the absence of the contaminant exceeding its reference value; total value of EBV, CMV, HHV-6 indexes is calculated, and if its value exceeds 1.0 and the blood chemical contaminant concentration is simultaneously increased, a secondary immunodeficient disease is diagnosed.

EFFECT: invention enables characterising the quantitative content of three viruses of a herpetic group in a human body simultaneously and assessing the combined effect of viral and chemical loads on the immunity.

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