FIELD: biotechnology.
SUBSTANCE: synthetic DNA encoding human IL-7 is obtained, its expression vector containing a strain of Chinese hamster ovary cells - recombinant human Interleukin-7 producer, deposited under number RKKK(P) 770D. Strain provides accumulation of up to 180 mg/l of IL-7 in the culture medium for 15 days in serum-free chemically defined medium. Also, a method for glycosylated human IL-7 purification from the culture medium is provided.
EFFECT: obtaining of a high-purity product with high glycosylation and specific activity.
5 cl, 7 dwg, 2 tbl, 5 ex
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