FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to biotechnology, in particular to method of obtaining interleukin-2 preparation, interleukin-2 preparation, obtained by said method, and pharmaceutical preparation, containing said interleukin-2 preparation. Saccharomyces cerevisiae strain - interleukin-2 producent is cultivated in nutrient medium for from 36 to 50 hours with aeration from 0.2 to 0.8 litres of air per minute per 100 litres of medium. Sediment of yeast cells is obtained by centrifugation or filtration. Cells are mechanically destructed for from 15 to 40 minutes. Sediment is purified from admixture yeast lipids and proteins by washing for from 15 to 40 minutes. Sediment is purified from admixture yeast lipids and proteins by washing with n-butanol. Target protein is extracted and its chromatographic purification is carried out.
EFFECT: inventions make it possible to obtain interleukin-2 preparation with high indices of purity, output and specific activity and apply it fin pharmaceutical immunomodulating compositions.
12 cl, 12 ex
| Title | Year | Author | Number |
|---|---|---|---|
| INTERLEUKIN-2 PREPARATION AND METHOD FOR ITS PREPARING | 2006 |
|
RU2304586C1 |
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| IMMUNOMODULATING COMPOSITION | 2006 |
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| STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING | 2006 |
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| PICHIA PASTORIS 2-2 YEAST STRAIN AS PRODUCER OF HUMAN PLATELET GROWTH FACTOR (PDGF-BB) AND METHOD FOR PRODUCTION OF HUMAN PLATELET GROWTH FACTOR | 2005 |
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