METHOD OF PREPARING OF HUMAN RECOMBINANT INTERLEUKIN-2 Russian patent published in 1995 - IPC

Abstract RU 2031125 C1

FIELD: biotechnology. SUBSTANCE: method of human recombinant interleukin-2 produced by yeast Saccharomyces cerevisiae from cultural medium of strain GRF 18-pJDB(ALPHOIL) (ВКПМ У-1038), involves the following general stages: growing of cell of strain GRF 18-pJDB(ALPHOIL) on the mineral medium with decreased content of inorganic phosphate, concentration of interleukin-2 by CM-Sephadex or CM-cellulose chromatography, purification of interleukin-2 by chromatography on the resin with reversed phase, lyophilic drying of interleukin-2 in the presence of mannitol. Specific biological activity of human recombinant interleukin-2 is 10-30 mln U/mg. Electrophoretic purity is above 90%, specific biological activity in the test for induction of cellular proliferation in strain CTLL-2 is 10-30 mln. IU/mg protein. EFFECT: increased activity and purification of the end product. 2 dwg

Similar patents RU2031125C1

Title	Year	Author	Number
RECOMBINANT PLASMID DNA PJ DB (MSIL), ENCODING OF HUMAN INTERLEUKIN-2 SYNTHESIS IN YEAST CELLS SACCHAROMYCES CEREVISIAE, METHOD OF ITS PREPARATION, AND YEAST STRAIN SACCHAROMYCES CEREVISIAE - A PRODUCER OF HUMAN INTERLEUKIN-2	0	Myasnikov Andrej Novomirovich Smirnov Mikhail Nikolaevich Avot Andris Yanovich Gren Elmar Yanovich Romanchikova Nadezhda Viktorovna Tsimanis Aleksandr Yurevich	SU1770359A1
YEAST STRAIN SACCHAROMYCES CEREVISIAE 1-60-D578 (MSIL) AS PRODUCER OF RECOMBINANT HUMAN INTERLEUKIN-2 AND METHOD FOR ITS PREPARING	2002	Smirnov M.N. Padkina M.V. Sambuk E.V.	RU2230781C1
METHOD OF PRODUCING RECOMBINANT PROTEIN, MODIFIED THROUGH ATTACHMENT OF HUMAN ALBUMIN FROM YEAST CULTURE FLUID	2007	Padkina Marina Vladimirovna Karabel'Skij Aleksandr Vladimirovich Zinov'Eva Julija Grigor'Evna Sambuk Elena Viktorovna Smirnov Mikhail Nikolaevich	RU2373286C2
METHOD FOR MAKING RECOMBINANT HUMAN ALPHA 16-INTERFERON AND PHARMACEUTICAL COMPOSITION FOR TREATING VIRAL DISEASES BASED ON RECOMBINANT HUMAN ALPHA 16-INTERFERON	2007	Padkina Marina Vladimirovna Dobrogorskaja Marina Vladimirovna Karabel'Skij Aleksandr Vladimirovich Sambuk Elena Viktorovna Smirnov Mikhail Nikolaevich	RU2380405C2
METHOD OF RECOMBINANT HUMAN INSULIN PREPARING	1999	Kalinin Ju.T. Urakov N.N. Ivanov V.T. Stepanov A.V. Miroshnikov A.I. Gavrikov V.G. Bairamashvili D.I. Donetskij I.A. Korobko V.G. Kostromina T.I. Guljaev V.A. Litvinenko M.A. Bajdus' A.N. Korobov V.I. Krasil'Nikov V.A. Borisov N.V. Shmatchenko N.A. Vorotnikova I.I. Gorkun O.G. Derbyshev V.I. Nozdrin V.N. Dunajtsev I.A. Lipin M.Ju. Bekmuratova I.I. Kobeleva V.A. Shepelin A.P. Martovetskij M.N. Fedjukin V.S. Abrosimova L.I.	RU2141531C1
METHOD FOR MANUFACTURING HUMAN RECOMBINATION LYMPHOTOXIN	1992	Denisov A.A. Torskij S.P. Nikolaeva O.G. Volozhantseva I.Ja. Urakov N.N. Korobko V.G.	RU2039823C1
INTERLEUKIN-2-BASE PHARMACEUTICAL PREPARATION (VARIANTS)	1998	Nikolaeva Z.K. Smirnov M.N. Jakovleva V.S.	RU2140283C1
METHOD OF PREPARING HUMAN RECOMBINANT TUMOR NECROSIS BETA-FACTOR	1997	Sinichkina S.A. Pustoshilova N.M. Masycheva V.I. Lebedev L.R. Korobko V.G.	RU2132385C1
STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING	2006	Padkina Marina Vladimirovna Zinov'Eva Julija Grigor'Evna Sambuk Elena Viktorovna Smirnov Mikhail Nikolaevich	RU2315105C1
GENETIC DESIGN OF pCXCRS-Fc FOR OBTAINING A RECOMBINANT MIXED PROTEIN WITH ANTI-IL-8 ACTIVITY	2017	Beletskij Igor Petrovich Glukhova Kseniya Alekseevna Ivanov Aleksej Alekseevich Lyashenko Alla Anatolevna Popova Olga Petrovna Prusakova Olga Vadimovna	RU2676317C1

RU 2 031 125 C1

Authors

Mjasnikov A.N.

Smirnov M.N.

Dates

1995-03-20—Published

1989-07-13—Filed