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2 290 434

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IPC

C12N1/19(2006-01-01)

C12N15/12(2006-01-01)

C12P21/02(2006-01-01)

C12R1/84(2006-01-01)

(21) (22)

Application

2005116851/13, 2005-06-02

(24)

Start date

2005-06-02

(22)

Actual filing date

2005-06-02

(45)

Published

2006-12-27

(72)

Inventor

Igonin Anton AlekseevichPal'Tseva Ekaterina MikhajlovnaUvarov Valentin Jur'EvichIvanov Aleksej AlekseevichSolov'Ev Valerij VladimirovichAkatov Vladimir SemenovichPrusakova Ol'Ga VadimovnaBeletskij Igor' Petrovich

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju

PICHIA PASTORIS 2-2 YEAST STRAIN AS PRODUCER OF HUMAN PLATELET GROWTH FACTOR (PDGF-BB) AND METHOD FOR PRODUCTION OF HUMAN PLATELET GROWTH FACTOR Russian patent published in 2006 - IPC C12N1/19 C12N15/12 C12P21/02 C12R1/84

Abstract RU 2290434 C1

FIELD: gene engineering, in particular preparation based on PDGF-BB, useful in therapy, veterinary, diagnosis, tissue culturing.

SUBSTANCE: invention relates to PDGF-BB production using Pichia pastoris 2-2 strain as producer of human platelet growth factor. Produced two-dimensional PDGF-BB form is characterized with purity of 95 %, specific activity of 3-5 ED₅₀/ng, and expression level in yeast up to 0.1 g/l of culture.

EFFECT: two-dimensional PDGF-BB form with high purity, specific activity and expression level in yeast.

5 cl, 11 dwg, 5 ex

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RU 2 290 434 C1

Authors

Igonin Anton Alekseevich

Pal'Tseva Ekaterina Mikhajlovna

Uvarov Valentin Jur'Evich

Ivanov Aleksej Alekseevich

Solov'Ev Valerij Vladimirovich

Akatov Vladimir Semenovich

Prusakova Ol'Ga Vadimovna

Beletskij Igor' Petrovich

Dates

2006-12-27—Published

2005-06-02—Filed