FIELD: gene engineering, in particular preparation based on PDGF-BB, useful in therapy, veterinary, diagnosis, tissue culturing.
SUBSTANCE: invention relates to PDGF-BB production using Pichia pastoris 2-2 strain as producer of human platelet growth factor. Produced two-dimensional PDGF-BB form is characterized with purity of 95 %, specific activity of 3-5 ED50/ng, and expression level in yeast up to 0.1 g/l of culture.
EFFECT: two-dimensional PDGF-BB form with high purity, specific activity and expression level in yeast.
5 cl, 11 dwg, 5 ex
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Year |
Author |
Number |
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