FIELD: biotechnology, cytokines, pharmacy.
SUBSTANCE: invention relates to a biotechnological method for preparing medicinal agents. Proposed method involves destruction of cells of yeast producer of interleukin-2 (IL-2) wherein synthesis of required protein (IL-2) is carried out, but this producer doesn't secrete this protein, and the following separation of water-insoluble fraction. In the process for preparing water-insoluble components of disturbed cells are retained. The interleukin-2 preparation obtained by this method shows less cost, it is active in oral administration and shows higher activity in some cases as compared with IL-2 preparations purified from components of yeast cells fraction.
EFFECT: improved preparing method, improved and valuable properties of interleukin-2.
13 cl, 2 tbl, 10 ex
| Title | Year | Author | Number |
|---|---|---|---|
| IMMUNOMODULATING COMPOSITION | 2006 |
|
RU2322452C2 |
| METHOD OF OBTAINING INTERLEUKIN-2 PREPARATION, INTERLEUKIN-2 PREPARATION AND PHARMACEUTICAL IMMUNOMODULATING PREPARATION | 2013 |
|
RU2565553C2 |
| PICHIA PASTORIS YEAST STRAIN - PRODUCER OF CHICKEN INTERFERON-GAMMA, RECOMBINANT PLASMID DNA WHICH FACILITATES BIOSYNTHESIS CHICKEN INTERFERON-GAMMA, AND CONSTRUCTION METHOD THEREOF | 2006 |
|
RU2386694C2 |
| YEAST STRAIN Pichia pastoris PS106(pHIG) AS PRODUCER OF HUMAN IMMUNE INTERFERON, RECOMBINANT PLASMID pHIG AND METHOD FOR ITS DESIGN | 2006 |
|
RU2315806C1 |
| METHOD OF PRODUCING SUSPENSIONS OF HYDROGEL MICROPARTICLES WITH GIVEN DIMENSIONS BASED ON RECOMBINANT COBWEB PROTEIN AND USE THEREOF | 2011 |
|
RU2478706C1 |
| METHOD OF PREPARING OF HUMAN RECOMBINANT INTERLEUKIN-2 | 1989 |
|
RU2031125C1 |
| METHOD OF PRODUCING RECOMBINANT PROTEIN, MODIFIED THROUGH ATTACHMENT OF HUMAN ALBUMIN FROM YEAST CULTURE FLUID | 2007 |
|
RU2373286C2 |
| STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING | 2006 |
|
RU2315105C1 |
| YEAST STRAIN SACCHAROMYCES CEREVISIAE 1-60-D578 (MSIL) AS PRODUCER OF RECOMBINANT HUMAN INTERLEUKIN-2 AND METHOD FOR ITS PREPARING | 2002 |
|
RU2230781C1 |
| METHOD OF PRODUCING RECOMBINANT SPIDER-WEB PROTEIN, FUSED PROTEIN, RECOMBINANT DNA, EXPRESSION VECTOR, HOST CELL AND PRODUCER STRAIN | 2010 |
|
RU2451023C1 |
