FIELD: biotechnology.
SUBSTANCE: said DNA aptamer binds to the bacteria Escherichia coli O157:H7 and is represented as a single-stranded DNA O157Flank with 81 nucleotides in length and molecular weight of 27 kDa. The DNA aptamer has high binding specificity relating to bacteria Escherichia coli O157:H7 and has primary sequence CATACGTTCGACTGCTACTCTGAGTCTGACCTTACAGTCAGTCAATCATGGGACAT ACGAAATGTGAGATGTACAGACTAG. The sequence of the DNA aptamer is selected as a result of positive selection rounds against bacteria Escherichia coli O157:H7 and negative selection against bacteria of other serogroups of Escherichia coli and closely related microorganisms using tRNA as competitor molecule for binding. The DNA aptamer is able to detect specifically the bacteria Escherichia coli O157:H7 in immunoaptamer PCR reaction at a concentration of 103 bacterial cells per 1 ml of solution.
EFFECT: invention enables to detect with high efficiency the said bacteria.
3 dwg, 2 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DNA SEQUENCE OF APTAMERS BINDING SHIGA-TOXIN OF TYPE 2 | 2014 |
|
RU2566552C1 |
| SEQUENCE OF DNA-APTAMERS, BINDING TO PROTEOLYTIC SUBUNIT OF NEUROTOXIN OF TYPE A CLOSTRIDIUM BOTULINUM | 2014 |
|
RU2571210C1 |
| DNA APTAMER SEQUENCE BINDING TO PEPTIDOGLYCAN-ASSOCIATED LIPOPROTEIN LEGIONELLA PNEUMOPHILA | 2019 |
|
RU2737829C1 |
| DNA SEQUENCE USED AS PROBE FOR PROVIDING MAXIMUM RATIO OF "SIGNAL/BACKGROUND" IN TEST SYSTEMS BASED ON IMMUNO-PCR | 2014 |
|
RU2566550C1 |
| METHOD TO DETECT AVAILABILITY OF BACTERIA Escherichia coli O157:H7 IN BIOLOGICAL AND FOOD SAMPLES BASED ON IMMUNODETECTION COUPLED WITH POLYMERASE CHAIN REACTION | 2014 |
|
RU2569196C1 |
| METHOD OF DIRECTED DEPOSIT OF OLIGONUCLEOTID LIBRARIES FOR THE REDUCTION OF NON-SPECIFIC ADSORPTION IN THE SOLID-PHASE SELECTION OF APTAMERS ON THE BASIS OF NUCLEIC ACIDS | 2015 |
|
RU2618872C1 |
| RECOMBINANT PLASMID DNA pGST/ART/X, CODING FUSED CHIMERIC POLYPEPTIDE GST/ART/X FOR HIGH SPECIFIC SELECTION OF APTAMERS TO TARGET PROTEIN X IN POLYPEPTIDE COMPOSITION | 2014 |
|
RU2558295C1 |
| METHOD FOR SPECIFIC SELECTION OF DNA MOLECULES (DNA APTAMERS) WITH AFFINITY FOR RECOMBINANT PROTEIN TARGET | 2012 |
|
RU2513700C1 |
| METHOD OF SELECTION APTAMERS TO SPECIFIED PROTEIN TARGETS ON CELL SURFACE | 2012 |
|
RU2518368C1 |
| METHOD FOR OBTAINING THERAPEUTIC AGENT FOR CURING OF TICK-BORNE ENCEPHALITIS | 2012 |
|
RU2501862C1 |
