FIELD: biotechnologies.
SUBSTANCE: studied culture of V. cholerae is grown up on Martin's agar, the bacterial suspension 1x109 mk/ml is prepared according to the turbidity standard of departmental standard specimens of Tarasevich State Research Institute, is added into two vessels with nutrient medium with the subsequent cultivation at 37°C. According to the special procedure the parameters of extracellular and intracellular producing of cadaverine are determined. The samples are studied in the capillary electrophoresis system Backman. The results are registered by computer electroforegramms with obtaining of parameters of samples at the time of detection of cadaverine. Using the formula the amount of the produced cadaverine in the studied samples is determined.
EFFECT: invention allows to assess by quantitative concentration of cadaverine the potential of Koch's bacilli at realisation of their adaptive functions and possibility to survive in various environments.
1 dwg, 3 tbl, 3 ex
