METHOD TO PRODUCE COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES Russian patent published in 2015 - IPC A23L1/30 A23D9/00 

FIELD: food industry.

SUBSTANCE: invention relates to food industry, in particular, to production of a composition used as a biologically active food additive, both independently in the form of finished pharma products of revitalizing and medical purpose for internal, external and parenteral application, and in food products. The method to produce a composition of biologically active substances with content from 0.01 to 50 wt % in the finished product includes introduction into milk fat, at plastic state temperature, of a concentrate of gram-positive anaerobic bacteria and powdered betulin, mixing to produce a homogeneous mass. At the same time the concentrate of gram-positive anaerobic bacteria is produced by connection of the cultivated dose of bifid bacteria culture with nutrient medium, such as hydrolysate-milk medium, at the following ratio, wt %: cultivated dose of bifid bacteria culture 0.06÷1.0, hydrolysate-milk medium - balance to 100%. Mixing and soaking for at least 12÷24 hours is carried out. On the basis of the milk fat, such as high-fat plastic cream, and betulin at 35÷41°C for 1 hour they prepare a fat dispersion at the following ratio, wt %: betulin 0.1÷2.0, high-fat plastic cream - balance to 100%. The produced fat dispersion is mixed with the concentrate of gram-positive anaerobic bacteria at the following ratio, wt %: fat dispersion 8.0÷10.0, concentrate of gram-positive anaerobic bacteria - balance to 100%. Then they mix it again for 0.3÷0.45 hours.

EFFECT: invention makes it possible to produce a composition, having high speed of bifid bacteria reproduction with their maximum growth, as well as long-term vitality of entire colony during further storage.

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