METHOD OF DETERMINING PINOSTROBIN OXIME IN BLOOD PLASMA Russian patent published in 2015 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: determination of pinostrobin oxime in blood plasma is performed by method of liquid chromato-mass-spectrometry with application of mixture of blood plasma with pinostrobin oxime and internal standard - substance, close by molecule structure to analysed substance. Separation of products is carried out by method of reversed-phase chromatography on column with size 4.6×100 mm, thermostated at 30°C, elution speed is 0.5 ml/min. As eluent applied is mixture of 10 mM of ammonium acetate and acetonitrile in ratio 10:90 respectively. Peak of pinostrobin oxime is detected by ion with m/z of 269.10, formed as a result of fragmentation of pinostrobin oxime ion with m/z of 286.10, and its concentration is calculated by formula C_oxime=2.073299×RS, where C_oxime is concentration of pinostrobin oxime (mcg/ml); RS is ratio of area of chromatographic peak of pinostrobin oxime to area of internal standard peak.

EFFECT: invention provides method for quantitative determination of pinostrobin oxime in blood plasma for application in experimental and clinic pharmacokinetics.

2 tbl, 6 dwg