METHOD FOR DETERMINING TOPIRAMATE IN BLOOD PLASMA Russian patent published in 2017 - IPC G01N33/48 

FIELD: pharmacology.

SUBSTANCE: quantitative determination of topiramate in human blood plasma using a highly selective chromatomass-spectrometry method is carried out. The analysis is carried out using a matrix of a solution of the internal standard, 2.3-4.5-bis-O-isopropylidene-beta-D-fructopyranose-N-(4-chlorobenzoyl) sulfamate and topiramate obtained by extraction from blood plasma. Separation of the extraction products is carried out on XTerra MS C18 column. Elution at a rate of 0.8 ml/min at a column temperature of 35°C is carried out with a solution A (10 mM of ammonium acetate) and solution B (a mixture of acetonitrile and 10 mM of ammonium acetate in the ratio of 90:10) in the ratio of solution A: solution B=40:60(%). Topiramate is detected by four daughter ions with m/z 95.9, 122.0, 161.9, 280.0, formed as a result of fragmentation of the parent molecular ion of topiramate with m/z 338.2, and its concentration is calculated by the formula: C=15.06732079×AR, where C is the concentration of topiramate (μg/ml), AR is the ratio of the area of the chromatographic peak of the topiramate to the peak area of the internal standard.

EFFECT: invention allows the quantitative determination of topiramate in the blood plasma of patients with high reliability.

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