METHOD OF DETERMINING CARNOSINE IN BIOLOGICAL MATERIALS Russian patent published in 2016 - IPC G01N33/52 G01N33/15 G01N30/04 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to experimental pharmacology, and can be used for quantitative determination of carnosine in tissues and physiological liquids. Determination of carnosine in biological materials is carried out by highly-selective mass spectrometry method using electrospray ionization. At that, deproteinization of blood plasma should be preliminary carried out using 10 % aqueous solution of trichloroacetic acid. Then aliquot of internal standard solution of L-alanyl-carnosine is added to deproteinizated sample. And separation of extraction products is performed at reversed-phase chromatographic column 4.6×150 mm with temperature separation of 35 °C and eluent feed rate 0.7 ml/min. Used eluent is 10 mM ammonium acetate, acidified with glacial acetic acid to pH 3.7, and mixture of acetonitrile with 10 mM ammonium acetate in ratio of 90:10, taken in ratio of 10:90, respectively. Detection of carnosine is carried out by four child ions with m/z 110.0, 156.1, 180.0, 210.1, formed as result of molecular ion carnosine disintegration with m/z 227.1. Concentration of carnosine is calculated by chromatographic peak carnosine area relation to L-alanyl-carnosine internal standard peak area.

EFFECT: invention provides highly selective and sensitive gas chromatography/mass-spectrometric method for quantitative determination of carnosine in biological substrates.

1 cl, 6 dwg, 2 tbl, 1 ex