FIELD: medicine.
SUBSTANCE: invention relates to field of examination and analysis of biological materials and deals with method for counting biological objects in sample and scanning cytometer on its basis. To realise counting of biological objects sample is placed in rectangular capillary, made with possibility of reflecting fluorescence signal from upper surface of rectangular capillary. Additional collection of fluorescence signal is provided by means of parabolic reflector, in focus of which analysed zone of capillary is placed, and replacement of analysed zones with biological objects is realised by moving capillary along its axis. Detected light flow is divided by wavelength and directed on first and second photomultipliers, output signals of which are proportional to light flow and fluorescence signal. From photomultipliers output signals are transmitted to amplitude and phase transformers for simultaneous processing and following multiplication of arrays of data, obtained from outlets of amplitude and phase transformers of signals in microprocessor.
EFFECT: technical result consists in increase of accuracy and simplification of measurement method, as well as in reduction of dimensions and increase of device mobility.
13 cl, 4 dwg
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Authors
Dates
2015-11-20—Published
2014-03-13—Filed