METHOD FOR PRODUCING A SALT OF Α-D-RIBOFURANOSO-1-PHOSPHATE OR Α-D-2-DEOXYRIBOFURANOSO-1-PHOSPHATE Russian patent published in 2019 - IPC C12P19/38 C12N9/10 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology and pharmaceutical chemistry. Disclosed is a method of producing a salt α-D-ribofuranose-1-phosphate and salt α-2-D-deoxyribofuranoso-1-phosphate, selected from a barium, magnesium, calcium, sodium or dicyclohexylamine salt. Enzymatic phosphorolysis of 7-methylguanosine hydroiodide salt or 7-methyl-2'-deoxyguanosine hydroiodide salt is performed in the presence of inorganic phosphate or dicyclohexylamine hydrophosphate, and pNP from Enterobacter or E. coli. Obtained α-D-ribofuranose-1-phosphate or α-D-2-deoxyribofuranoso-1-phosphate is reacted with a water-ammonia solution of Ba²⁺, Mg²⁺ or Ca²⁺ acetate to obtain corresponding salt. To obtain sodium salt of dicyclohexylamine salt α-D-ribofuranose-1-phosphate or α-D-2-deoxyribofuranoso-1-phosphate is dissolved in water and ion-exchange resin Dowex-50 in Na⁺ form is added to solution.

EFFECT: invention provides stereo-specificity of the reaction, complete conversion of the starting compounds and avoids the use of large excess reagents, yield of the desired compounds is 74–96 %.

1 cl, 4 dwg, 10 ex