LIPID PARTICLES CONTAINING NUCLEIC ACIDS AND RELATED METHODS Russian patent published in 2016 - IPC C12N15/87 C12N15/09 A61K9/127 A61K48/00 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biochemistry. Claimed are: lipid particle for delivery of nucleic acid (versions), method of introducing nucleic acid in cell, method of producing lipid particles, which include nucleic acid. Lipid particle contains core, consisting of nucleic acid and cation lipid, and secondary lipids, surrounding said core. In other version lipid particle contains core, consisting of nucleic acid, cation lipid and secondary lipids, and secondary lipids, surrounding said core. Method of introduction of nucleic acid into cell includes bringing cell in contact with said lipid particles. Method of producing lipid particles, containing nucleic acid, includes introduction of first flow, which contains nucleic acid, and introduction of second flow, which contains substances, forming lipid particle, into device providing flowing of first and second flows, flowing first and second flows from first part of device into second part of device, mixing flows in second part of device to provide third flow, containing lipid particles.

EFFECT: inventions provide improvement of lipid particles, containing therapeutic substance, increased efficiency of encapsulation of nucleic acids into lipid particle.

18 cl, 33 dwg, 1 tbl, 5 ex