CHEMICAL AGENT ENABLING INACTIVATING MICROORGANISMS, EXTRACTING AND PRESERVING BACTERIAL DNA IN FORM APPLICABLE FOR HIGH-EFFICACY MOLECULAR DIAGNOSTICS Russian patent published in 2016 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to a chemical agent for preliminary preparation of a biological sample for diagnosing in vitro an infectious disease. The chemical agent for preliminary preparation of a biological sample for diagnosing in vitro an infectious disease by PCR technique represents an aqueous solution containing alcohol, alkali and tertiary amine in the concentrations promoting higher amplification efficacy for at least 2 cycles. A method for preliminary preparation of a biological sample. A method for storing the biological sample. Using the chemical agent for preliminary preparation of the biological sample.

EFFECT: using the claimed chemical agent enables cleaning the cells effectively from PCR inhibitors, inactivating microorganisms in biological samples, diluting them though preserving DNA stability and promoting higher amplification for at least 2 cycles.

5 cl, 8 tbl, 1 ex