PHARMACOLOGICAL COMBINATION OF POLYCATIONIC CARRIER PEG-PEI-TAT WITH INCLUDED IN IT PLASMID CARRYING THERAPEUTIC GENES HSVtk AND GM-CSF FOR GENE THERAPY OF TUMOROUS DISEASES Russian patent published in 2016 - IPC A61K48/00 A61K47/48 A61K31/708 C12N15/85 C07K14/535 A61P35/00 

FIELD: bioengineering.

SUBSTANCE: medical anticancer drug is produced, it contains the gene structure in the form of a plasmide DNA, comprising genes of the thymidine kinase of the herpes simplex virus (HSVtk), and a granulocytic-macrophage colony-stimulating factor (GM-CSF), included in a polymer carrier - copolymer PEG-PEI-TAT peptide. Wherein the gene structure ensures the expression of both therapeutic genes HSVtk and GM-CSF from the same vector. The gene structure is intended for intratumoural introduction under the mode of the progressively increased single dose calculated depending on the tumour size, with the further injection of the prodrug - ganciclovir.

EFFECT: invention ensures the effective delivery and diffusion in the tumour cells of the plasmid pCMV-HSVtk-GM-CSF, and ensures with high efficiency the selective destruction of cancer cells preventing further metastasis.

8 cl, 9 dwg, 16 tbl, 7 ex