GENE THERAPY PREPARATION FCU1-BSFM/PP FOR TREATING SOLID TUMOURS Russian patent published in 2024 - IPC C12N15/85 C12N15/54 C12N15/55 C12N15/62 A61K31/711 A61K31/513 A61K47/34 A61K48/00 A61P35/00 

Abstract RU 2824977 C1

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to genetic engineering. Disclosed is a bicistronic plasmid construct FCU1-BsFm, a method for preparing the anti-tumour preparation FCU1-BsFm/PP, method of its delivery, methods of tumour cell replication inhibition and treatment of malignant tumours. Plasmid construct FCU1-BsFm encodes a chimeric gene of yeast cytosine deaminase and uracilphosphoribosyltransferase and a gene for secreted mutant flagellin Bacillus subtilis. To obtain a preparation FCU1-BsFm/PP, the plasmid structure is “packaged” in a carrier based on polyethylene glycol, polyethyleneimine and TAT-peptide. Method of treating malignant tumours involves intratumoural administration of the preparation FCU1-BsFm/PP in combination with the introduction of prodrug 5-fluorocytosine. Expression of the FCU1-BsFm construct in the tumour focus provides inhibition of tumour cell replication. Tumours represent sarcoma, colon tumour, lung adenocarcinoma, cervical cancer, breast cancer, ovarian adenocarcinoma or squamous cell carcinoma of skin.

EFFECT: inventions provide an effective method of delivering plasmid DNA into a tumour focus and inside cells, provide an anti-tumour effect mediated through a cytotoxic and immunostimulating effect.

6 cl, 24 dwg, 5 tbl, 9 ex

Similar patents RU2824977C1

Title Year Author Number
MULTI-PURPOSE PROMOTER, EXPRESSION VECTOR, AND METHOD OF SELECTIVE KILLING CANCER CELLS WITH THEIR USE 2012
  • Alekseenko Irina Vasil'Evna
  • Vinogradova Tat'Jana Viktorovna
  • Demidjuk Il'Ja Valer'Evich
  • Kostrov Sergej Viktorovich
  • Pleshkan Viktor Viktorovich
  • Chernov Igor' Pavlovich
  • Mitjaev Maksim Vjacheslavovich
  • Zinov'Eva Marina Valer'Evna
  • Georgiev Georgij Pavlovich
  • Sobolev Aleksandr Sergeevich
  • Rozenkrants Andrej Aleksandrovich
  • Ulasov Aleksej Valentinovich
  • Kuz'Min Denis Vladimirovich
  • Khramtsov Jurij Viktorovich
  • Kopantsev Evgenij Pavlovich
  • Uspenskaja Natalija Jakovlevna
  • Kostina Marija Borisovna
  • Monastyrskaja Galina Sergeevna
  • Sverdlov Evgenij Davidovich
RU2476596C1
MULTIPURPOSE CANCER-SPECIFIC PROMOTERS AND USING THEM IN ANTI-CANCER THERAPY 2013
  • Sverdlov Evgenij Davidovich
  • Alekseenko Irina Vasil'Evna
  • Vinogradova Tat'Jana Viktorovna
  • Kopantsev Evgenij Pavlovich
  • Korobko Igor' Viktorovich
  • Kostina Marija Borisovna
  • Monastyrskaja Galina Sergeevna
  • Pleshkan Viktor Viktorovich
  • Sass Aleksandr Viktorovich
  • Uspenskaja Natalija Jakovlevna
  • Chernov Igor' Pavlovich
RU2539764C2
PHARMACOLOGICAL COMBINATION OF POLYCATIONIC CARRIER PEG-PEI-TAT WITH INCLUDED IN IT PLASMID CARRYING THERAPEUTIC GENES HSVtk AND GM-CSF FOR GENE THERAPY OF TUMOROUS DISEASES 2013
  • Alekseenko Irina Vasil'Evna
  • Vinogradova Tat'Jana Viktorovna
  • Zinov'Eva Marina Valer'Evna
  • Kopantsev Evgenij Pavlovich
  • Monastyrskaja Galina Sergeevna
  • Kostina Marija Borisovna
  • Snezhkov Evgenij Valer'Evich
  • Chernov Igor' Pavlovich
  • Pleshkan Viktor Viktorovich
  • Kostrov Sergej Viktorovich
  • Potapov Viktor Kuz'Mich
  • Bezborodova Ol'Ga Alekseevna
  • Nemtsova Elena Romanovna
  • Jakubovskaja Raisa Ivanovna
  • Uspenskaja Natalija Jakovlevna
  • Rozenkrants Andrej Aleksandrovich
  • Sobolev Aleksandr Sergeevich
  • Ulasov Aleksej Valentinovich
  • Khramtsov Jurij Viktorovich
  • Sverdlov Evgenij Davidovich
RU2575077C2
DRUG COMBINATION FOR GENE-IMMUNE THERAPY 2022
  • Alekseenko Irina Vasilevna
  • Sverdlov Evgenij Davidovich
  • Koksharov Maksim Olegovich
  • Snezhkov Evgenij Valerevich
RU2792683C1
POXVIRAL ONCOLYTIC VECTORS 2008
  • Ehrbs Filipp
  • Folopp Zhoann
RU2503717C2
POX VIRAL ONCOLYTIC VECTORS 2008
  • Ehrbs Filipp
  • Folopp Zhoann
RU2557312C2
POXVIRAL ONCOLYTIC VECTORS 2008
  • Ehrbs Filipp
  • Folopp Zhoann
RU2508401C2
RECOMBINANT PLASMID DNA pGEM-Puro-DS-Apo COMPRISING SYNTHETIC APOPTIN GENE FLANKED BY SEQUENCES OF VACCINIA VIRUS GENOME OF ISLAND C10L-C12L, AND RECOMBINANT STRAIN VVdGF-ApoS24/2 OF VACCINIA VIRUS PRODUCING APOPTIN 2012
  • Chumakov Petr Mikhajlovich
  • Kochneva Galina Vadimovna
  • Babkina Irina Nikolaevna
  • Lupan Tat'Jana Anatol'Evna
  • Grazhdantseva Antonina Anatol'Evna
  • Judin Pavel Valer'Evich
  • Sivolobova Galina Filippovna
  • Popov Evgenij Gennad'Evich
  • Netesov Sergej Viktorovich
RU2492238C1
UNIVERSAL PROMOTER FOR EXPRESSION OF THERAPEUTIC GENES IN MAMMALIAN CELLS 2013
  • Sverdlov Evgenij Davidovich
  • Alekseenko Irina Vasil'Evna
  • Bezborodova Ol'Ga Alekseevna
  • Vinogradova Tat'Jana Viktorovna
  • Kashkin Kirill Nikitich
  • Kopantsev Evgenij Pavlovich
  • Monastyrskaja Galina Sergeevna
  • Nemtsova Elena Romanovna
  • Uspenskaja Natal'Ja Jakovlevna
  • Chernov Igor' Pavlovich
  • Jakubovskaja Raisa Ivanovna
RU2551784C1
RECOMBINANT PLASMID pCpG-CytDA/upp FOR EXPRESSION OF FUSION PROTEIN CYTOSINE DEAMINASE-URASILPHOSPHORIBOSYL TRANSFERASE, RECOMBINANT PLASMID pCpG-CytDA/upp/VP22 FOR EXPRESSION OF FUSION PROTEIN CYTOSINE DEAMINASE-URASILPHOSPHORIBOSYL TRANSFERASE-VP22, THERAPEUTIC COMPOSITION FOR THERAPY OF CANCEROUS DISEASES AND METHOD FOR USE THEREOF 2012
  • Karshieva Saida Shamilevna
  • Krasikova Ljudmila Sergeevna
  • Beljavskij Aleksandr Vadimovich
RU2580220C2

RU 2 824 977 C1

Authors

Sukhikh Gennadij Tikhonovich

Ashrafyan Levon Andreevich

Alekseenko Irina Vasilevna

Kondrateva Sofiya Alekseevna

Kuzmich Aleksej Ivanovich

Pleshkan Viktor Viktorovich

Yudin Sergej Mikhajlovich

Makarov Valentin Vladimirovich

Keskinov Artur Arturovich

Yudin Vladimir Sergeevich

Makarova Anna Sergeevna

Ershov Pavel Viktorovich

Dates

2024-08-19Published

2023-03-03Filed