FERMENTATION MEDIUM AND METHOD OF PRODUCING RECOMBINANT PROTEINS Russian patent published in 2015 - IPC C12N1/14 C12N1/20 C12N9/00 C12P1/02 C12P1/04 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and specifically to a fermentation medium and a method of producing recombinant proteins using said medium. A fermentation medium for producing recombinant proteins selected from a group including GM-CSF, streptokinase and lipase, using microorganisms selected from a group including: E. Coli, Streptomyces sp. and Rhizomucor sp., is characterised by keeping concentration of urea or derivatives thereof in the range of 0.5 g/l to 2 g/l. The medium contains, per litre of water, basic salts in the following amounts: orthophosphoric acid (85%) 2.67-133.5 ml, calcium sulphate 0.093-4.65 g, potassium sulphate 1.82-91 g, magnesium sulphate-7H₂O 1.49-74.5 g, potassium hydroxide 0.413-20.65 g, glycerine 4-200 g. The medium contains, per litre of water, trace elements in the following amounts: copper sulphate-5H₂O 0.6-30 g, sodium iodide 0.008-0.4 g, manganese sulphate-H₂O 0.3-15 g, sodium molybdate-H₂O 0.02-1 g, boric acid 0.002-0.1 g, cobalt chloride 0.05-2.5 g, zinc chloride 2-100 g, iron (II) sulphate-7H₂O 6.5-325 g, biotin 0.02-1 g, sulphuric acid 0.5-25 ml.

EFFECT: invention increases the output of end products.

6 cl, 27 dwg, 3 tbl, 16 ex