FIELD: medicine.
SUBSTANCE: present invention refers to biotechnology, more specifically to recombinant production of antibodies, and is applicable for reducing antibody heterogeneity during culturing. There are producing low-heterogeneity antibodies caused by variations of the relative contents of a lysine residue on C-terminals by adding divalent zinc ions (Zn+2) in the concentration of 0.05-1.5 mM to a culture medium during the culturing procedure and reducing an osmotic pressure of the cell culture medium from 240 to 260 mOsm/kg.
EFFECT: invention enables producing the high-quality monoclonal antibodies as compared to a standard technology by reducing a portion of primary variants in the antibodies having the C-terminal variation of lysine that reduces the heterogeneity in antibodies.
15 cl, 12 dwg, 12 tbl, 12 ex
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| METHOD OF PRODUCTION PROTEINS USING COMPOUNDS THAT PREVENT AGING | 2006 |
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